Western Blot:Article Title: Dual-Synergistic Nanomodulator Alleviates Exosomal PD-L1 Expression Enabling Exhausted Cytotoxic T Lymphocytes Rejuvenation for Potentiated iRFA-Treated Hepatocellular Carcinoma Immunotherapy
Article Snippet: DyLight 680-conjugated fluorescent secondary antibodies were supplied by Cell Signaling Technology. .. Furthermore, essential reagents for western blotting, including RIPA lysis buffer, phosphatase inhibitor cocktail, WB transfer buffer, and QuickBlock blocking buffer, were obtained from Beyotime Biotechnology Inc. Tricolor Prestained Protein Ladder, PAGE gel rapid preparation kits (10% and 12.5%), were purchased from Epizyme Biomedical Technology Co., Ltd. Nuclear staining reagents, Hoechst 33342 and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride), were sourced from Solarbio Science & Technology Co., Ltd. Bromethol was acquired from EasyCheck Nanjing AIBI Biotechnology Co., Ltd., and the laboratory essentials, Triton X-100 and 4% paraformaldehyde, were purchased from Biosharp Biotechnology Co., Ltd. .. APC/Cyanine7 anti-mouse CD45 Antibody, Brilliant Violet 421 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, PerCP/Cyanine5.5 anti-mouse CD3 Antibody, True-Nuclear Mouse Treg Flow Kit featuring FOXP3 APC, PE anti-mouse NK-1.1 Antibody, Brilliant Violet 510 anti-mouse CD4 Antibody, PE anti-mouse/human CD11b Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody, and Brilliant Violet 421 antimouse CD335 (NKp46) Antibody were all sourced from BioLegend.
Lysis:Article Title: Dual-Synergistic Nanomodulator Alleviates Exosomal PD-L1 Expression Enabling Exhausted Cytotoxic T Lymphocytes Rejuvenation for Potentiated iRFA-Treated Hepatocellular Carcinoma Immunotherapy
Article Snippet: DyLight 680-conjugated fluorescent secondary antibodies were supplied by Cell Signaling Technology. .. Furthermore, essential reagents for western blotting, including RIPA lysis buffer, phosphatase inhibitor cocktail, WB transfer buffer, and QuickBlock blocking buffer, were obtained from Beyotime Biotechnology Inc. Tricolor Prestained Protein Ladder, PAGE gel rapid preparation kits (10% and 12.5%), were purchased from Epizyme Biomedical Technology Co., Ltd. Nuclear staining reagents, Hoechst 33342 and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride), were sourced from Solarbio Science & Technology Co., Ltd. Bromethol was acquired from EasyCheck Nanjing AIBI Biotechnology Co., Ltd., and the laboratory essentials, Triton X-100 and 4% paraformaldehyde, were purchased from Biosharp Biotechnology Co., Ltd. .. APC/Cyanine7 anti-mouse CD45 Antibody, Brilliant Violet 421 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, PerCP/Cyanine5.5 anti-mouse CD3 Antibody, True-Nuclear Mouse Treg Flow Kit featuring FOXP3 APC, PE anti-mouse NK-1.1 Antibody, Brilliant Violet 510 anti-mouse CD4 Antibody, PE anti-mouse/human CD11b Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody, and Brilliant Violet 421 antimouse CD335 (NKp46) Antibody were all sourced from BioLegend.
Blocking Assay:Article Title: Dual-Synergistic Nanomodulator Alleviates Exosomal PD-L1 Expression Enabling Exhausted Cytotoxic T Lymphocytes Rejuvenation for Potentiated iRFA-Treated Hepatocellular Carcinoma Immunotherapy
Article Snippet: DyLight 680-conjugated fluorescent secondary antibodies were supplied by Cell Signaling Technology. .. Furthermore, essential reagents for western blotting, including RIPA lysis buffer, phosphatase inhibitor cocktail, WB transfer buffer, and QuickBlock blocking buffer, were obtained from Beyotime Biotechnology Inc. Tricolor Prestained Protein Ladder, PAGE gel rapid preparation kits (10% and 12.5%), were purchased from Epizyme Biomedical Technology Co., Ltd. Nuclear staining reagents, Hoechst 33342 and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride), were sourced from Solarbio Science & Technology Co., Ltd. Bromethol was acquired from EasyCheck Nanjing AIBI Biotechnology Co., Ltd., and the laboratory essentials, Triton X-100 and 4% paraformaldehyde, were purchased from Biosharp Biotechnology Co., Ltd. .. APC/Cyanine7 anti-mouse CD45 Antibody, Brilliant Violet 421 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, PerCP/Cyanine5.5 anti-mouse CD3 Antibody, True-Nuclear Mouse Treg Flow Kit featuring FOXP3 APC, PE anti-mouse NK-1.1 Antibody, Brilliant Violet 510 anti-mouse CD4 Antibody, PE anti-mouse/human CD11b Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody, and Brilliant Violet 421 antimouse CD335 (NKp46) Antibody were all sourced from BioLegend.
Polyacrylamide Gel Electrophoresis:Article Title: Dual-Synergistic Nanomodulator Alleviates Exosomal PD-L1 Expression Enabling Exhausted Cytotoxic T Lymphocytes Rejuvenation for Potentiated iRFA-Treated Hepatocellular Carcinoma Immunotherapy
Article Snippet: DyLight 680-conjugated fluorescent secondary antibodies were supplied by Cell Signaling Technology. .. Furthermore, essential reagents for western blotting, including RIPA lysis buffer, phosphatase inhibitor cocktail, WB transfer buffer, and QuickBlock blocking buffer, were obtained from Beyotime Biotechnology Inc. Tricolor Prestained Protein Ladder, PAGE gel rapid preparation kits (10% and 12.5%), were purchased from Epizyme Biomedical Technology Co., Ltd. Nuclear staining reagents, Hoechst 33342 and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride), were sourced from Solarbio Science & Technology Co., Ltd. Bromethol was acquired from EasyCheck Nanjing AIBI Biotechnology Co., Ltd., and the laboratory essentials, Triton X-100 and 4% paraformaldehyde, were purchased from Biosharp Biotechnology Co., Ltd. .. APC/Cyanine7 anti-mouse CD45 Antibody, Brilliant Violet 421 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, PerCP/Cyanine5.5 anti-mouse CD3 Antibody, True-Nuclear Mouse Treg Flow Kit featuring FOXP3 APC, PE anti-mouse NK-1.1 Antibody, Brilliant Violet 510 anti-mouse CD4 Antibody, PE anti-mouse/human CD11b Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody, and Brilliant Violet 421 antimouse CD335 (NKp46) Antibody were all sourced from BioLegend.
Staining:Article Title: Dual-Synergistic Nanomodulator Alleviates Exosomal PD-L1 Expression Enabling Exhausted Cytotoxic T Lymphocytes Rejuvenation for Potentiated iRFA-Treated Hepatocellular Carcinoma Immunotherapy
Article Snippet: DyLight 680-conjugated fluorescent secondary antibodies were supplied by Cell Signaling Technology. .. Furthermore, essential reagents for western blotting, including RIPA lysis buffer, phosphatase inhibitor cocktail, WB transfer buffer, and QuickBlock blocking buffer, were obtained from Beyotime Biotechnology Inc. Tricolor Prestained Protein Ladder, PAGE gel rapid preparation kits (10% and 12.5%), were purchased from Epizyme Biomedical Technology Co., Ltd. Nuclear staining reagents, Hoechst 33342 and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride), were sourced from Solarbio Science & Technology Co., Ltd. Bromethol was acquired from EasyCheck Nanjing AIBI Biotechnology Co., Ltd., and the laboratory essentials, Triton X-100 and 4% paraformaldehyde, were purchased from Biosharp Biotechnology Co., Ltd. .. APC/Cyanine7 anti-mouse CD45 Antibody, Brilliant Violet 421 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, PerCP/Cyanine5.5 anti-mouse CD3 Antibody, True-Nuclear Mouse Treg Flow Kit featuring FOXP3 APC, PE anti-mouse NK-1.1 Antibody, Brilliant Violet 510 anti-mouse CD4 Antibody, PE anti-mouse/human CD11b Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody, and Brilliant Violet 421 antimouse CD335 (NKp46) Antibody were all sourced from BioLegend.
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